WB
Western blot analysis of various lysates using TFAM Rabbit mAb at 1:4000 dilution incubatedovernight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB
Western blot analysis of lysates from Rat testis using TFAM Rabbit mAb at 1:4000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing TFAM Rabbit mAb at a dilutionof 1:5500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedHuman colon cancer tissue using TFAMRabbit mAb (dilution 1:200) followedby a further incubation with Cy3 Goat AntiRabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining(Blue). Objective: 40x. Perform high pressureantigen retrieval with 0.01 M citrate buffer(pH 6.0) prior to IF staining.IF-P
Confocal imaging of paraffin-embeddedHuman kidney tissue using TFAM Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPI wasused for nuclear staining (Blue). Objective:40x. Perform high pressure antigen retrievalwith 0.01 M citrate buffer (pH 6.0) prior to IFstaining.| Product Name | TFAM Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:4000-1:16000 IF-P:1:200-1:800 IHC:1:2000-1:8000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFAM |
|---|---|
| Gene Synonyms | TCF6 MTTF1 MTTFA TCF6L1 TCF6L2 TCF6L3 MTDPS15 |
| Gene Full Name | transcription factor A, mitochondrial |
| Gene Summary | This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| Alternative Names | TCF6 MTTF1 MTTFA TCF6L1 TCF6L2 TCF6L3 MTDPS15 TFAM |
| Molecular Weight(MW) | 29kDa(Observed band size:24kDa) |
| Cellular Localization | Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid. |
WB
Western blot analysis of various lysates using TFAM Rabbit mAb at 1:4000 dilution incubatedovernight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
WB
Western blot analysis of lysates from Rat testis using TFAM Rabbit mAb at 1:4000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing TFAM Rabbit mAb at a dilutionof 1:5500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedHuman colon cancer tissue using TFAMRabbit mAb (dilution 1:200) followedby a further incubation with Cy3 Goat AntiRabbit IgG (H+L) (dilution 1:500)(Red). DAPI was used for nuclear staining(Blue). Objective: 40x. Perform high pressureantigen retrieval with 0.01 M citrate buffer(pH 6.0) prior to IF staining.
IF-P
Confocal imaging of paraffin-embeddedHuman kidney tissue using TFAM Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPI wasused for nuclear staining (Blue). Objective:40x. Perform high pressure antigen retrievalwith 0.01 M citrate buffer (pH 6.0) prior to IFstaining.| Application Notes | WB:1:4000-1:16000 IF-P:1:200-1:800 IHC:1:2000-1:8000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
