WB
Western blot analysis of various lysates, using [KD Validated] CD98/SLC3A2 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.WB
Western blot analysis of lysates from wild type (WT) and CD98/SLC3A2 knockdown (KD) HeLa cells using[KD Validated] CD98/SLC3A2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.ICC/IF
Confocal imaging of HeLa cells using [KDValidated] CD98/SLC3A2 Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3-conjugated Goat AntiRabbit IgG (H+L) (dilution 1:500)(Red). The cells were counterstained with αTubulin mAb (dilution 1:400) followedby incubation with ABflo® 488-conjugatedGoat Anti-Mouse IgG (H+L) (dilution1:500) (Green). DAPI was used for nuclearstaining (blue). Objective: 100x.ICC/IF
Confocal imaging of Hep G2 cells using [KDValidated] CD98/SLC3A2 Rabbit mAb(,dilution 1:200) followed by a furtherincubation with Cy3-conjugated Goat AntiRabbit IgG (H+L) (dilution 1:500)(Red). The cells were counterstained with αTubulin mAb (dilution 1:400) followedby incubation with ABflo® 488-conjugatedGoat Anti-Mouse IgG (H+L) (dilution1:500) (Green). DAPI was used for nuclearstaining (blue). Objective: 100x.FC
Flow cytometry: 1X10^6 SH-SY5Y cells (LowExpression,left) and HeLa cells (right) weresurface-stained with CD98/SLC3A2 RabbitmAb (2 μg/mL,orange line) orABflo® 647 Rabbit IgG isotype control(5 μl/Test,blue line), followed byAlexa Fluor® 647 conjugated goat antirabbit pAb staining. Non-fluorescentlystained cells were used as blank control (redline).FC
Flow cytometry: 1X10^6 HeLa cells weresurface-stained with ABflo® 647 Rabbit IgGisotype control (5 μl/Test,left) orCD98/SLC3A2 Rabbit mAb (2μg/mL,right).| Product Name | [KD Validated] CD98/SLC3A2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000-1:4000 ICC/IF:1:200-1:2000 FC:1:500-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC3A2 |
|---|---|
| Gene Synonyms | 4F2 CD98 MDU1 4F2HC 4T2HC NACAE CD98HC |
| Gene Full Name | solute carrier family 3 member 2 |
| Gene Summary | This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. [provided by RefSeq, Nov 2010] |
| Alternative Names | 4F2 CD98 MDU1 4F2HC 4T2HC NACAE CD98HC CD98/SLC3A2 |
| Molecular Weight(MW) | 57/61/67/68/71kDa(Observed band size:75-120kDa) |
| Cellular Localization | Apical cell membrane, cell membrane, single-pass type II membrane protein, cell junction, lysosome membrane, melanosome, basolateral cell membrane. |
WB
Western blot analysis of various lysates, using [KD Validated] CD98/SLC3A2 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.
WB
Western blot analysis of lysates from wild type (WT) and CD98/SLC3A2 knockdown (KD) HeLa cells using[KD Validated] CD98/SLC3A2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.
ICC/IF
Confocal imaging of HeLa cells using [KDValidated] CD98/SLC3A2 Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3-conjugated Goat AntiRabbit IgG (H+L) (dilution 1:500)(Red). The cells were counterstained with αTubulin mAb (dilution 1:400) followedby incubation with ABflo® 488-conjugatedGoat Anti-Mouse IgG (H+L) (dilution1:500) (Green). DAPI was used for nuclearstaining (blue). Objective: 100x.
ICC/IF
Confocal imaging of Hep G2 cells using [KDValidated] CD98/SLC3A2 Rabbit mAb(,dilution 1:200) followed by a furtherincubation with Cy3-conjugated Goat AntiRabbit IgG (H+L) (dilution 1:500)(Red). The cells were counterstained with αTubulin mAb (dilution 1:400) followedby incubation with ABflo® 488-conjugatedGoat Anti-Mouse IgG (H+L) (dilution1:500) (Green). DAPI was used for nuclearstaining (blue). Objective: 100x.
FC
Flow cytometry: 1X10^6 SH-SY5Y cells (LowExpression,left) and HeLa cells (right) weresurface-stained with CD98/SLC3A2 RabbitmAb (2 μg/mL,orange line) orABflo® 647 Rabbit IgG isotype control(5 μl/Test,blue line), followed byAlexa Fluor® 647 conjugated goat antirabbit pAb staining. Non-fluorescentlystained cells were used as blank control (redline).
FC
Flow cytometry: 1X10^6 HeLa cells weresurface-stained with ABflo® 647 Rabbit IgGisotype control (5 μl/Test,left) orCD98/SLC3A2 Rabbit mAb (2μg/mL,right).| Application Notes | WB:1:1000-1:4000 ICC/IF:1:200-1:2000 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
