WB
Western blot analysis of various lysates using Syndecan 1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue usingCD138/Syndecan-1 Rabbit mAb at adilution of 1:5000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedHuman tonsil tissue usingCD138/Syndecan-1 Rabbit mAb (dilution 1:100) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPIwas used for nuclear staining (Blue). Highpressure antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Product Name | CD138/Syndecan-1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:2000 IF-P:1:100-1:1000 IHC:1:2000-1:8000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SDC1 |
|---|---|
| Gene Synonyms | SDC CD138 SYND1 syndecan |
| Gene Full Name | syndecan 1 |
| Gene Summary | The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008] |
| Alternative Names | SDC CD138 SYND1 syndecan CD138/Syndecan-1 |
| Molecular Weight(MW) | 32kDa(Observed band size:90-100/180-250kDa) |
| Cellular Localization | Membrane, Secreted, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using Syndecan 1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissue usingCD138/Syndecan-1 Rabbit mAb at adilution of 1:5000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedHuman tonsil tissue usingCD138/Syndecan-1 Rabbit mAb (dilution 1:100) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPIwas used for nuclear staining (Blue). Highpressure antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Application Notes | WB:1:1000-1:2000 IF-P:1:100-1:1000 IHC:1:2000-1:8000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
