WB
Western blot analysis of various lysates using ASC/TMS1 Rabbit mAb at 1:3000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): JurkatExposure time: 30s.ICC/IF
Confocal imaging of THP-1 cellsusing ASC/TMS1 Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500)(Red).DAPI wasused for nuclear staining (Blue). Objective:100x.IF-P
Confocal imaging of paraffin-embeddedMouse spleen using ASC/TMS1 Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500)(Red). DAPI wasused for nuclear staining (Blue). Objective:40x. Perform high pressure antigen retrievalwith 0.01 M citrate buffer (pH 6.0) prior to IFstaining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissueusing ASC/TMS1 Rabbit mAb at adilution of 1:200 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse intestin tissueusing ASC/TMS1 Rabbit mAb at adilution of 1:200 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.| Product Name | ASC/TMS1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:3000-1:18000 ICC/IF:1:50-1:200 IHC:1:200-1:2000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pycard |
|---|---|
| Gene Synonyms | Asc TNS1 masc CARD5 TMS-1 9130417A21Rik |
| Gene Full Name | PYD and CARD domain containing |
| Gene Summary | Enables identical protein binding activity and protein dimerization activity. Involved in several processes, including positive regulation of cellular component organization; positive regulation of cytokine production; and regulation of defense response. Acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macrophage cytokine production; and regulation of autophagy. Located in cytosol; extracellular region; and nucleus. Part of NLRP6 inflammasome complex. Is expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. Orthologous to human PYCARD (PYD and CARD domain containing). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Asc TNS1 masc CARD5 TMS-1 9130417A21Rik ASC/TMS1 |
| Molecular Weight(MW) | 21kDa(Observed band size:17/24kDa) |
| Cellular Localization | Cytoplasm, Nucleus, Mitochondrion, Inflammasome. |
WB
Western blot analysis of various lysates using ASC/TMS1 Rabbit mAb at 1:3000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): JurkatExposure time: 30s.
ICC/IF
Confocal imaging of THP-1 cellsusing ASC/TMS1 Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500)(Red).DAPI wasused for nuclear staining (Blue). Objective:100x.
IF-P
Confocal imaging of paraffin-embeddedMouse spleen using ASC/TMS1 Rabbit mAb(dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500)(Red). DAPI wasused for nuclear staining (Blue). Objective:40x. Perform high pressure antigen retrievalwith 0.01 M citrate buffer (pH 6.0) prior to IFstaining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon tissueusing ASC/TMS1 Rabbit mAb at adilution of 1:200 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse intestin tissueusing ASC/TMS1 Rabbit mAb at adilution of 1:200 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:3000-1:18000 ICC/IF:1:50-1:200 IHC:1:200-1:2000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
