WB
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilutionat 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IF-F
Application: IHC-Fr. Species: Mouse. Site: colon. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TMS1 / ASC antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TMS1 / ASC antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | TMS1 / ASC Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse TMS1 / ASC aa 1-193. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IF-F:1:500 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PYCARD |
|---|---|
| Gene Synonyms | ASC TMS TMS1 CARD5 TMS-1 |
| Gene Full Name | PYD and CARD domain containing |
| Gene Summary | This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Apoptosis associated speck like protein containing a CARD antibody Apoptosis-associated speck-like protein containing a CARD antibody ASC antibody CARD 5 antibody CARD5 antibody Caspase recruitment domain containing protein 5 antibody Caspase recruitment domain protein 5 antibody Caspase recruitment domain-containing protein 5 antibody hASC antibody MGC10332 antibody Apoptosis associated speck like protein containing a CARD antibody Apoptosis-associated speck-like protein containing a CARD antibody ASC antibody CARD 5 antibody CARD5 antibody Caspase recruitment domain containing protein 5 antibody Caspase recruitment domain protein 5 antibody Caspase recruitment domain-containing protein 5 antibody hASC antibody MGC10332 antibody PYCARD antibody PYD and CARD domain containing antibody PYD and CARD domain containing protein antibody PYD and CARD domain-containing protein antibody Target of methylation induced silencing 1 antibody Target of methylation-induced silencing 1 antibody TMS 1 antibody TMS antibody TMS1 antibody |
| Molecular Weight(MW) | 21 kDa(Observed band size: 25 kDa) |
| Cellular Localization | Cytoplasm, Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus. |
WB
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of TMS1 / ASC on different lysates with Rabbit anti-TMS1 / ASC antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilutionat 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IF-F
Application: IHC-Fr. Species: Mouse. Site: colon. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TMS1 / ASC antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TMS1 / ASC antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000 IF-F:1:500 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
