WB
Western blot analysis of various lysates using ASC/TMS1 Rabbit pAb at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): Jurkat. Exposure time: 5 s.| Product Name | ASC/TMS1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 - 1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYCARD |
|---|---|
| Gene Synonyms | ASC TMS TMS1 CARD5 TMS-1 |
| Gene Full Name | PYD and CARD domain containing |
| Gene Summary | This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ASC TMS TMS1 CARD5 TMS-1 ASC/TMS1 Asc TNS1 masc CARD5 TMS-1 9130417A21Rik ASC/TMS1 |
| Molecular Weight(MW) | 15 kDa/20 kDa/22 kDa kDa(Observed band size: 17 kDa/24 kDa) |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus.Inflammasome . |
WB
Western blot analysis of various lysates using ASC/TMS1 Rabbit pAb at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): Jurkat. Exposure time: 5 s.| Application Notes | WB:1:5000 - 1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS, pH 7.3, containing 50% glycerol. Preserved with Proclin300 or sodium azide. May contain 0.05% BSA as specified on the Certificate of Analysis. |
