WB
Western blot analysis of various lysates using LI Cadherin/Cadherin-17 Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.IF-P
Immunofluorescence analysis of paraffinembedded mouse Intestine using LICadherin/Cadherin-17 Rabbit mAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IF-P
Immunofluorescence analysis of paraffinembedded rat rectum using LICadherin/Cadherin-17 Rabbit mAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing LI Cadherin/Cadherin-17 Rabbit mAb at a dilution of 1:400 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse colon tissue using LICadherin/Cadherin-17 Rabbit mAb ata dilution of 1:400 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat intestine tissue using LICadherin/Cadherin-17 Rabbit mAb ata dilution of 1:400 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.| Product Name | LI Cadherin/Cadherin-17 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:2000 IHC:1:400-1:1600 IF-P:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH17 |
|---|---|
| Gene Synonyms | HPT1 CDH16 HPT-1 |
| Gene Full Name | cadherin 17 |
| Gene Summary | This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009] |
| Alternative Names | HPT1 CDH16 HPT-1 LI Cadherin/Cadherin-17 |
| Molecular Weight(MW) | 92kDa(Observed band size:120kDa) |
| Cellular Localization | Cell membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using LI Cadherin/Cadherin-17 Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
IF-P
Immunofluorescence analysis of paraffinembedded mouse Intestine using LICadherin/Cadherin-17 Rabbit mAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IF-P
Immunofluorescence analysis of paraffinembedded rat rectum using LICadherin/Cadherin-17 Rabbit mAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing LI Cadherin/Cadherin-17 Rabbit mAb at a dilution of 1:400 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse colon tissue using LICadherin/Cadherin-17 Rabbit mAb ata dilution of 1:400 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat intestine tissue using LICadherin/Cadherin-17 Rabbit mAb ata dilution of 1:400 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.| Application Notes | WB:1:1000-1:2000 IHC:1:400-1:1600 IF-P:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
