WB
Western blot analysis of various lysates using PIGR Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB
Western blot analysis of various lysates using PIGR Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.ICC/IF
Immunofluorescence analysis of A-431 cellsusing PIGR Rabbit pAb at dilution of1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of HeLa cellsusing PIGR Rabbit pAb at dilution of1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using PIGR Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer using PIGR Rabbit pAb at dilution of 1:100 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.| Product Name | PIGR Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIGR |
|---|---|
| Gene Full Name | polymeric immunoglobulin receptor |
| Gene Summary | This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009] |
| Alternative Names | PIGR |
| Molecular Weight(MW) | 83kDa(Observed band size:100kDa) |
| Cellular Localization | Cell membrane, Secreted, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates using PIGR Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
WB
Western blot analysis of various lysates using PIGR Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of A-431 cellsusing PIGR Rabbit pAb at dilution of1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing PIGR Rabbit pAb at dilution of1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using PIGR Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer using PIGR Rabbit pAb at dilution of 1:100 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
