WB
Western blot analysis of various lysates using EEF1D Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.ICC/IF
Immunofluorescence analysis of HeLa cellsusing EEF1D Rabbit pAb at dilutionof 1:200 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing EEF1D Rabbit pAb at dilutionof 1:200 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human liver tissue using EEF1DRabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using EEF1DRabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney tissue using EEF1DRabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.IP
Immunoprecipitation of EEF1D in 300 µg extracts from jurkat cells using 3 µg EEF1D Rabbit pAb.Western blot analysis was performed using EEF1D Rabbit pAb at 1:500 dilution.IP
Immunoprecipitation of EEF1D in 300 µg extracts from Jurkat cells using 3 µg EEF1D Rabbit pAb .Western blot analysis was performed using EEF1D Rabbit pAb at 1:500 dilution.| Product Name | EEF1D Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EEF1D |
|---|---|
| Gene Synonyms | EF1D EF-1D FP1047 NEDTCHAL |
| Gene Full Name | eukaryotic translation elongation factor 1 delta |
| Gene Summary | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010] |
| Alternative Names | EF1D EF-1D FP1047 EEF1D |
| Molecular Weight(MW) | 31kDa(Observed band size:40/35kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of various lysates using EEF1D Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of HeLa cellsusing EEF1D Rabbit pAb at dilutionof 1:200 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing EEF1D Rabbit pAb at dilutionof 1:200 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver tissue using EEF1DRabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain tissue using EEF1DRabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney tissue using EEF1DRabbit pAb at a dilution of 1:200(40x lens). High pressure antigen retrievalwas performed with 0.01 M citrate buffer (pH6.0) prior to IHC staining.
IP
Immunoprecipitation of EEF1D in 300 µg extracts from jurkat cells using 3 µg EEF1D Rabbit pAb.Western blot analysis was performed using EEF1D Rabbit pAb at 1:500 dilution.
IP
Immunoprecipitation of EEF1D in 300 µg extracts from Jurkat cells using 3 µg EEF1D Rabbit pAb .Western blot analysis was performed using EEF1D Rabbit pAb at 1:500 dilution.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
