WB
Western blot analysis of lysates from Mouse lung using LYVE1 Rabbit mAb at 1:13000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of lysates from Rat liver using LYVE1 Rabbit mAb at 1:13000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse intestin tissue using LYVE1Rabbit mAb at a dilution of 1:6000(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedMouse liver tissue using LYVE1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Product Name | LYVE1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:10000-1:20000 IHC:1:2000-1:8000 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Lyve1 |
|---|---|
| Gene Synonyms | Xlkd1 Lyve-1 Crsbp-1 1200012G08Rik |
| Gene Full Name | lymphatic vessel endothelial hyaluronan receptor 1 |
| Gene Summary | Enables cargo receptor activity; hyaluronic acid binding activity; and transmembrane signaling receptor activity. Involved in hyaluronan catabolic process and receptor-mediated endocytosis. Acts upstream of or within glycosaminoglycan catabolic process. Located in plasma membrane. Is expressed in several structures, including alimentary system; cardiovascular system; hemolymphoid system; meninges; and metanephros. Orthologous to human LYVE1 (lymphatic vessel endothelial hyaluronan receptor 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Xlkd1 Lyve-1 Crsbp-1 1200012G08Rik Lyve1 |
| Molecular Weight(MW) | 34kDa(Observed band size:55-70kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of lysates from Mouse lung using LYVE1 Rabbit mAb at 1:13000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of lysates from Rat liver using LYVE1 Rabbit mAb at 1:13000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse intestin tissue using LYVE1Rabbit mAb at a dilution of 1:6000(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-EDTA Buffer (pH9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedMouse liver tissue using LYVE1 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Application Notes | WB:1:10000-1:20000 IHC:1:2000-1:8000 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
