WB
Western blot analysis of lysates from wild type (WT) and MCT1 knockdown (KD) 293T cells using [KDValidated] MCT1 Rabbit mAb at 1:2000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.ICC/IF
Confocal imaging of HeLa cells using [KDValidated] MCT1 Rabbit mAb (dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (AS007, dilution 1:500) (Red). Thecells were counterstained with α-TubulinMouse mAb (dilution 1:400) followedby incubation with ABflo® 488-conjugatedGoat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used fornuclear staining (Blue). Objective: 100x.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing [KD Validated] MCT1 Rabbit mAb at a dilution of 1:600 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.IF-P
Confocal imaging of paraffin-embeddedHuman colon cancer tissue using [KDValidated] MCT1 Rabbit mAb (dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPIwas used for nuclear staining (Blue). Highpressure antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Product Name | [KD Validated] MCT1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:2000-1:8000 IHC:1:200-1:800 ICC/IF:1:200-1:800 IF-P:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC16A1 |
|---|---|
| Gene Synonyms | MCT HHF7 MCT1 MCT1D |
| Gene Full Name | solute carrier family 16 member 1 |
| Gene Summary | The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009] |
| Alternative Names | MCT HHF7 MCT1 MCT1D |
| Molecular Weight(MW) | 54kDa(Observed band size:43kDa) |
| Cellular Localization | apical plasma membrane, basal plasma membrane, , cell junction, centrosome, extracellular exosome, lateral plasma membrane, plasma membrane, Cell membrane, Multi-pass membrane protein. |
WB
Western blot analysis of lysates from wild type (WT) and MCT1 knockdown (KD) 293T cells using [KDValidated] MCT1 Rabbit mAb at 1:2000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
ICC/IF
Confocal imaging of HeLa cells using [KDValidated] MCT1 Rabbit mAb (dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (AS007, dilution 1:500) (Red). Thecells were counterstained with α-TubulinMouse mAb (dilution 1:400) followedby incubation with ABflo® 488-conjugatedGoat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used fornuclear staining (Blue). Objective: 100x.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing [KD Validated] MCT1 Rabbit mAb at a dilution of 1:600 (40x lens).High pressure antigen retrieval performedwith 0.01M Tris-EDTA Buffer (pH 9.0) prior toIHC staining.
IF-P
Confocal imaging of paraffin-embeddedHuman colon cancer tissue using [KDValidated] MCT1 Rabbit mAb (dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPIwas used for nuclear staining (Blue). Highpressure antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Application Notes | WB:1:2000-1:8000 IHC:1:200-1:800 ICC/IF:1:200-1:800 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
