WB
Western blot analysis of lysates from Rat brain, using BMPR1B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.WB
Western blot analysis of various lysates using BMPR1B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of L929 cellsusing BMPR1B Rabbit pAb at dilutionof 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human mammary cancer usingBMPR1B Rabbit pAb at dilution of1:200 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat ovary using BMPR1B Rabbit pAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney using BMPR1BRabbit pAb at dilution of 1:300 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.| Product Name | BMPR1B Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BMPR1B |
|---|---|
| Gene Synonyms | ALK6 AMD3 AMDD BDA2 ALK-6 BDA1D CDw293 |
| Gene Full Name | bone morphogenetic protein receptor type 1B |
| Gene Summary | This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Alternative Names | ALK6 AMD3 AMDD BDA2 ALK-6 BDA1D CDw293 BMPR1B |
| Molecular Weight(MW) | 57kDa(Observed band size:51kDa) |
| Cellular Localization | Membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of lysates from Rat brain, using BMPR1B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.
WB
Western blot analysis of various lysates using BMPR1B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of L929 cellsusing BMPR1B Rabbit pAb at dilutionof 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human mammary cancer usingBMPR1B Rabbit pAb at dilution of1:200 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat ovary using BMPR1B Rabbit pAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney using BMPR1BRabbit pAb at dilution of 1:300 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
