WB
Western blot analysis of various lysates using NT5E/CD73 Rabbit mAb at 1:160000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): Raji, RAW 264.7Exposure time: 45 s.IF-P
Confocal imaging of paraffin-embeddedMouse brain tissue using NT5E/CD73 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Microwave antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue usingNT5E/CD73 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human spleen tissue usingNT5E/CD73 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue usingNT5E/CD73 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.| Product Name | NT5E/CD73 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:80000-1:160000 IF-P:1:50-1:200 IHC:1:300-1:3000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NT5E |
|---|---|
| Gene Synonyms | NT eN NT5 NTE eNT CD73 E5NT CALJA |
| Gene Full Name | 5'-nucleotidase ecto |
| Gene Summary | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| Alternative Names | NT eN NT5 NTE eNT CD73 E5NT CALJA |
| Molecular Weight(MW) | 57/63kDa(Observed band size:70kDa) |
| Cellular Localization | Cell surface, cytosol, external side of plasma membrane, extracellular exosome, nucleoplasm, plasma membrane, cell membrane, GPI-anchor, Lipid-anchor. |
WB
Western blot analysis of various lysates using NT5E/CD73 Rabbit mAb at 1:160000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): Raji, RAW 264.7Exposure time: 45 s.
IF-P
Confocal imaging of paraffin-embeddedMouse brain tissue using NT5E/CD73 RabbitmAb (dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).Microwave antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis tissue usingNT5E/CD73 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human spleen tissue usingNT5E/CD73 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue usingNT5E/CD73 Rabbit mAb at adilution of 1:300 (40x lens). High pressureantigen retrieval performed with 0.01MCitrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:80000-1:160000 IF-P:1:50-1:200 IHC:1:300-1:3000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
