WB
Western blot analysis of various lysates, using LAMP3 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using LAMP3 Rabbit pAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHCstaining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart using LAMP3 RabbitpAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.+| Product Name | LAMP3 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LAMP3 |
|---|---|
| Gene Synonyms | LAMP CD208 DCLAMP LAMP-3 TSC403 DC LAMP DC-LAMP |
| Gene Full Name | lysosomal associated membrane protein 3 |
| Gene Summary | Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM, Dec 2010] |
| Alternative Names | LAMP CD208 DCLAMP LAMP-3 TSC403 DC LAMP DC-LAMP LAMP3 |
| Molecular Weight(MW) | 44kDa(Observed band size:70kDa) |
| Cellular Localization | Cytoplasmic vesicle membrane, Lysosome membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of various lysates, using LAMP3 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using LAMP3 Rabbit pAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHCstaining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart using LAMP3 RabbitpAb at dilution of 1:200 (40x lens).Microwave antigen retrieval performed with0.01M PBS Buffer (pH 7.2) prior to IHC staining.+| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
