WB
Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody at 1/2000 dilution and competitor's antibody at 1/1000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: Jurkat cell lysate (15 µg/Lane), Lane 3: NIH/3T3 cell lysate (15 µg/Lane), Lane 4: C2C12 cell lysate (15 µg/Lane), Lane 5: C6 cell lysate (15 µg/Lane), Lane 6: PC-12 cell lysate (15 µg/Lane), Exposure time: 6 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution and competitor's antibody at 1/1000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody at 1/2000 dilution. Lane 1: HeLa whole cell lysate, Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling PARP1 with Rabbit anti-PARP1 antibody at 1/500 dilution. Cells were fixed in 100% methanol for 5 minutes, blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PARP1 antibody at 1/500 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/800 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PARP1 antibody at 1/4000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PARP1 antibody at 1/4000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-PARP1 antibody at 1/4000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Application: IF-Tissue, Species: Human, Site: breast cancer, Sample: Paraffin-embedded section, Antibody concentration: 1/1000.FC
Flow cytometric analysis of HeLa cells labeling PARP1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | PARP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within N-terminal human PARP1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:2000 IHC:1:4000 ICC/IF:1:500 FC:1:1000 IF-P:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PARP1 |
|---|---|
| Gene Synonyms | PARP PARS PPOL ADPRT ARTD1 ADPRT1 PARP-1 ADPRT 1 pADPRT-1 Poly-PARP |
| Gene Full Name | poly(ADP-ribose) polymerase 1 |
| Gene Summary | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008] |
| Alternative Names | ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP ribosyltransferase 1 antibody ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP ribosyltransferase 1 antibody NAD(+) ADP-ribosyltransferase 1 antibody pADPRT 1 antibody pADPRT1 antibody PARP 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly (ADP ribose) polymerase 1 antibody poly (ADP ribose) polymerase family member 1 antibody Poly [ADP-ribose] polymerase 1 antibody Poly(ADP ribose) polymerase antibody poly(ADP ribose) synthetase antibody poly(ADP ribosyl)transferase antibody Poly[ADP ribose] synthetase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody |
| Molecular Weight(MW) | 113kDa(Observed band size:113kDa) |
| Cellular Localization | Nucleus, nucleolus, chromosome. |
WB
Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody at 1/2000 dilution and competitor's antibody at 1/1000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane), Lane 2: Jurkat cell lysate (15 µg/Lane), Lane 3: NIH/3T3 cell lysate (15 µg/Lane), Lane 4: C2C12 cell lysate (15 µg/Lane), Lane 5: C6 cell lysate (15 µg/Lane), Lane 6: PC-12 cell lysate (15 µg/Lane), Exposure time: 6 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution and competitor's antibody at 1/1000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody at 1/2000 dilution. Lane 1: HeLa whole cell lysate, Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling PARP1 with Rabbit anti-PARP1 antibody at 1/500 dilution. Cells were fixed in 100% methanol for 5 minutes, blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PARP1 antibody at 1/500 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/800 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PARP1 antibody at 1/4000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PARP1 antibody at 1/4000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-PARP1 antibody at 1/4000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Application: IF-Tissue, Species: Human, Site: breast cancer, Sample: Paraffin-embedded section, Antibody concentration: 1/1000.
FC
Flow cytometric analysis of HeLa cells labeling PARP1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:4000 ICC/IF:1:500 FC:1:1000 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
