WB
Western blot analysis of RelB on different lysates with Rabbit anti-RelB antibody at 1/1000 dilution. Lane 1: Raji cell lysate, Lane 2: Daudi cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RelB antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | RelB Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human RelB aa 1-50 / 579. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RELB |
|---|---|
| Gene Synonyms | IREL I-REL IMD53 REL-B |
| Gene Full Name | RELB proto-oncogene, NF-kB subunit |
| Gene Summary | Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and protein kinase binding activity. Involved in lymphocyte differentiation and negative regulation of interferon-beta production. Located in several cellular components, including centrosome; chromatin; and nucleoplasm. Part of nucleus and transcription repressor complex. Implicated in breast cancer and immunodeficiency 53. Biomarker of breast cancer and transitional cell carcinoma. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | I REL antibody I-Rel antibody IREL antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody relB antibody RELB_HUMAN antibody Reticuloendotheliosis viral oncogene homolog B antibody Transcription factor Rel B antibody Transcription factor RelB antibody v rel avian reticuloendotheliosis viral oncogene homolog B antibody I REL antibody I-Rel antibody IREL antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody relB antibody RELB_HUMAN antibody Reticuloendotheliosis viral oncogene homolog B antibody Transcription factor Rel B antibody Transcription factor RelB antibody v rel avian reticuloendotheliosis viral oncogene homolog B antibody v rel reticuloendotheliosis viral oncogene homolog B antibody |
| Molecular Weight(MW) | 62kDa(Observed band size:62kDa) |
| Cellular Localization | Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
WB
Western blot analysis of RelB on different lysates with Rabbit anti-RelB antibody at 1/1000 dilution. Lane 1: Raji cell lysate, Lane 2: Daudi cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RelB antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
