WB
Western blot analysis of Histone H4 (acetyl K5) on different lysates with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, Lane 4: C6 cell lysate, Lane 5: C6 treated with 1μM TSA for 18 hours cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L ( 594) was used as the secondary antibody at 1/1000 dilution.ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L ( 594) was used as the secondary antibody at 1/1000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence staining of paraffin- embedded rat brain tissue using anti-Histone H4(acetyl K5) antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with Histone H4 (acetyl K5) Recombinant Rabbit Monoclonal Antibody at 1/300 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H4 (acetyl K5) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Product Name | Histone H4 (acetyl K5) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Histone H4 aa 1-50 / 103 (acetyl K5). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 IF-P:1:50-1:400 ChIP:Use 0.5~2 μg for 25 μg of chromatin. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4FA HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Alternative Names | Histone gene cluster 1 H4A antibody Histone gene cluster 2 H4 antibody dJ160A22.1 antibody dJ160A22.2 antibody dJ221C16.1 antibody dJ221C16.9 antibody FO108 antibody H4 antibody H4 histone family member A antibody H4 histone family member B antibody Histone gene cluster 1 H4A antibody Histone gene cluster 2 H4 antibody dJ160A22.1 antibody dJ160A22.2 antibody dJ221C16.1 antibody dJ221C16.9 antibody FO108 antibody H4 antibody H4 histone family member A antibody H4 histone family member B antibody H4 histone family member C antibody H4 histone family member D antibody H4 histone family member E antibody H4 histone family member G antibody H4 histone family member H antibody H4 histone family member I antibody H4 histone family member J antibody H4 histone family member K antibody H4 histone family member M antibody H4 histone family member N antibody H4 histone family 2 antibody H4/A antibody H4/B antibody H4/C antibody H4/D antibody H4/E antibody H4/G antibody H4/H antibody H4/I antibody H4/J antibody H4/K antibody H4/M antibody H4/N antibody H4/O antibody H4/p antibody H4_HUMAN antibody H4F2 antibody H4F2iii antibody H4F2iv antibody H4FA antibody H4FB antibody H4FC antibody H4FD antibody H4FE antibody H4FG antibody H4FH antibody H4FI antibody H4FJ antibody H4FK antibody HIST1 cluster H4A antibody HIST1 cluster H4B antibody HIST1 cluster H4D antibody HIST2H4 antibody Hist4 cluster H4 antibody Hist4h4 antibody histone 1 H4a antibody histone 1 H4c antibody histone 1 H4d antibody histone 1 H4f antibody histone 1 H4h antibody histone 1 H4i antibody histone 1 H4j antibody histone 1 H4k antibody histone 1 H4l antibody histone 2 H4a antibody histone 2 H4b antibody Histone 4 family member M antibody histone 4 H4 antibody histone cluster 1 H4 antibody histone cluster 1 H4a antibody histone cluster 1 H4b antibody histone cluster 1 H4c antibody histone cluster 1 H4d antibody histone cluster 1 H4e antibody histone cluster 1 H4f antibody histone cluster 1 H4h antibody histone cluster 1 H4i antibody histone cluster 1 H4j antibody histone cluster 1 H4k antibody histone cluster 1 H4l antibody histone cluster 2 H4a antibody histone cluster 2 H4b antibody histone cluster 4 H4 antibody Histone family member A antibody Histone family member B antibody Histone family member D antibody Histone family member H antibody Histone family member I antibody Histone family member L antibody Histone gene cluster 1 H4 antibody Histone gene cluster 1 H4D antibody Histone gene cluster 1 H4E antibody Histone gene cluster 1 H4K antibody Histone gene cluster 4 H4 antibody Histone gene cluster 4 H4 histone antibody Histone H4 antibody histone IV family 2 antibody H4K5ac antibody |
| Molecular Weight(MW) | 11kDa(Observed band size:11kDa) |
| Cellular Localization | Nucleus, Chromosome. |
WB
Western blot analysis of Histone H4 (acetyl K5) on different lysates with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, Lane 4: C6 cell lysate, Lane 5: C6 treated with 1μM TSA for 18 hours cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin ( red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L ( 594) was used as the secondary antibody at 1/1000 dilution.
ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling Histone H4 (acetyl K5) with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L ( 594) was used as the secondary antibody at 1/1000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Histone H4 (acetyl K5) antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 (acetyl K5) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence staining of paraffin- embedded rat brain tissue using anti-Histone H4(acetyl K5) antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with Histone H4 (acetyl K5) Recombinant Rabbit Monoclonal Antibody at 1/300 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H4 (acetyl K5) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:100-1:500 IF-P:1:50-1:400 ChIP:Use 0.5~2 μg for 25 μg of chromatin. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
