WB
Western blot analysis of SIRT2 on Human brain tissue lysates with Rabbit anti-SIRT2 antibody at 1/1000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of SIRT2 on different lysates with Rabbit anti-SIRT2 antibody at 1/1000 dilution. Lane 1: MCF7 cell lysate, Lane 2: HeLa cell lysate, Lane 3: HEK-293 cell lysate, Lane 4: LNCaP cell lysate, Lane 5: Jurkat cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SIRT2 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SIRT2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human SIRT2 aa 335-389 / 389. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:2000 IHC:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT2 |
|---|---|
| Gene Synonyms | SIR2 SIR2L SIR2L2 |
| Gene Full Name | sirtuin 2 |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010] |
| Alternative Names | FLJ35621 antibody FLJ37491 antibody NAD dependent deacetylase sirtuin 2 antibody NAD-dependent deacetylase sirtuin-2 antibody NAD-dependent protein deacetylase sirtuin-2 antibody Regulatory protein SIR2 homolog 2 antibody Silencing information regulator 2 like antibody Silent information regulator 2 antibody SIR2 antibody SIR2 like protein 2 antibody FLJ35621 antibody FLJ37491 antibody NAD dependent deacetylase sirtuin 2 antibody NAD-dependent deacetylase sirtuin-2 antibody NAD-dependent protein deacetylase sirtuin-2 antibody Regulatory protein SIR2 homolog 2 antibody Silencing information regulator 2 like antibody Silent information regulator 2 antibody SIR2 antibody SIR2 like protein 2 antibody Sir2 related protein type 2 antibody SIR2 S. cerevisiae homolog-loke 2 antibody SIR2-like protein 2 antibody SIR2L antibody SIR2L2 antibody SIRT2 antibody SIRT2_HUMAN antibody Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody Sirtuin 2 antibody Sirtuin type 2 antibody |
| Molecular Weight(MW) | 43kDa(Observed band size:40/35kDa) |
| Cellular Localization | Nucleus, Cytoplasm, Midbody, Chromosome, Cell projection, Myelin membrane. |
WB
Western blot analysis of SIRT2 on Human brain tissue lysates with Rabbit anti-SIRT2 antibody at 1/1000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of SIRT2 on different lysates with Rabbit anti-SIRT2 antibody at 1/1000 dilution. Lane 1: MCF7 cell lysate, Lane 2: HeLa cell lysate, Lane 3: HEK-293 cell lysate, Lane 4: LNCaP cell lysate, Lane 5: Jurkat cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SIRT2 antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:2000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
