WB
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody at 1/5000 dilution and competitor's antibody at 1/1000 dilution. Lane 1: 293T cell lysate, Lane 2: A549 cell lysate, Lane 3: HeLa cell lysate, Lane 4: A-172 cell lysate, Lane 5: MCF7 cell lysate (negative), Lane 6: C2C12 cell lysate, Lane 7: C6 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes 6 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody at 1/5000 dilution. Lane 1: 293T-si NT cell lysate (10 µg/Lane), Lane 2: 293T-si N Cadherin cell lysate (10 µg/Lane), Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IF-F
Application: IHC-Fr, Species: Mouse, Site: Liver, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.IF-F
Application: IHC-Fr, Species: Mouse, Site: Liver, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-N Cadherin antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-N Cadherin antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-N Cadherin antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | N Cadherin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human N Cadherin aa 161-210 / 906. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000-1:20000 IF-F:1:500-1:1000 IHC:1:1000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH2 |
|---|---|
| Gene Synonyms | KIP1 MEN4 CDKN4 MEN1B P27KIP1 |
| Gene Full Name | cyclin dependent kinase inhibitor 1B |
| Gene Summary | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014] |
| Alternative Names | CADH2_HUMAN antibody Cadherin 2 antibody Cadherin 2 N cadherin neuronal antibody Cadherin 2 type 1 antibody Cadherin 2 type 1 N cadherin neuronal antibody Cadherin 2 type 1 N-cadherin (neuronal) antibody Cadherin-2 antibody Cadherin2 antibody Calcium dependent adhesion protein neuronal antibody CD325 antibody CADH2_HUMAN antibody Cadherin 2 antibody Cadherin 2 N cadherin neuronal antibody Cadherin 2 type 1 antibody Cadherin 2 type 1 N cadherin neuronal antibody Cadherin 2 type 1 N-cadherin (neuronal) antibody Cadherin-2 antibody Cadherin2 antibody Calcium dependent adhesion protein neuronal antibody CD325 antibody CD325 antigen antibody CDH2 antibody CDHN antibody CDw325 antibody CDw325 antigen antibody N cadherin 1 antibody N-cadherin antibody NCAD antibody Neural cadherin antibody OTTHUMP00000066304 antibody OTTHUMP00000067378 antibody |
| Molecular Weight(MW) | 100kDa(Observed band size:140-150kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody at 1/5000 dilution and competitor's antibody at 1/1000 dilution. Lane 1: 293T cell lysate, Lane 2: A549 cell lysate, Lane 3: HeLa cell lysate, Lane 4: A-172 cell lysate, Lane 5: MCF7 cell lysate (negative), Lane 6: C2C12 cell lysate, Lane 7: C6 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes 6 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody at 1/5000 dilution. Lane 1: 293T-si NT cell lysate (10 µg/Lane), Lane 2: 293T-si N Cadherin cell lysate (10 µg/Lane), Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IF-F
Application: IHC-Fr, Species: Mouse, Site: Liver, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
IF-F
Application: IHC-Fr, Species: Mouse, Site: Liver, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-N Cadherin antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-N Cadherin antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-N Cadherin antibody at 1/5000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:5000-1:20000 IF-F:1:500-1:1000 IHC:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
