WB
Western blot analysis of Raptor on different lysates with Rabbit anti-Raptor antibody at 1/2000 dilution. Lane 1: 293T cell lysate (30 µg/Lane), Lane 2: K-562 cell lysate (30 µg/Lane), Lane 3: Neuro-2a cell lysate (30 µg/Lane), Lane 4: Mouse brain tissue lysate (40 µg/Lane), Lane 5: Rat brain tissue lysate (40 µg/Lane), Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Raptor antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Raptor Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Raptor aa 1286-1335 / 1335. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPTOR |
|---|---|
| Gene Synonyms | KOG1 Mip1 |
| Gene Full Name | regulatory associated protein of MTOR complex 1 |
| Gene Summary | This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Alternative Names | KIAA1303 antibody KOG1 antibody Mip1 antibody P150 target of rapamycin (TOR) scaffold protein antibody p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody P150 target of rapamycin (TOR)-scaffold protein antibody Raptor antibody Regulatory associated protein of mTOR antibody Regulatory associated protein of MTOR complex 1 antibody Regulatory-associated protein of mTOR antibody KIAA1303 antibody KOG1 antibody Mip1 antibody P150 target of rapamycin (TOR) scaffold protein antibody p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody P150 target of rapamycin (TOR)-scaffold protein antibody Raptor antibody Regulatory associated protein of mTOR antibody Regulatory associated protein of MTOR complex 1 antibody Regulatory-associated protein of mTOR antibody RPTOR antibody RPTOR_HUMAN antibody |
| Molecular Weight(MW) | 149kDa(Observed band size:149kDa) |
| Cellular Localization | Lysosome membrane, Cytoplasm, Cytoplasmic granule. |
WB
Western blot analysis of Raptor on different lysates with Rabbit anti-Raptor antibody at 1/2000 dilution. Lane 1: 293T cell lysate (30 µg/Lane), Lane 2: K-562 cell lysate (30 µg/Lane), Lane 3: Neuro-2a cell lysate (30 µg/Lane), Lane 4: Mouse brain tissue lysate (40 µg/Lane), Lane 5: Rat brain tissue lysate (40 µg/Lane), Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Raptor antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
