WB
Western blot analysis of delta 1 Catenin/CAS on SK-Br-3 cell lysates with Rabbit anti-delta 1 Catenin/CAS antibody at 1/1000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-delta 1 Catenin/CAS antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Application: IF-Tissue, Species: Human, Site: liver cancer, Sample: Paraffin-embedded section, Antibody concentration: 1/500.| Product Name | delta 1 Catenin/CAS Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human delta 1 Catenin/CAS aa 850-950 (C terminal). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000 IHC:1:500-1:2000 IF-P:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTNND1 |
|---|---|
| Gene Synonyms | CAS p120 BCDS2 CTNND P120CAS P120CTN p120(CAS) p120(CTN) |
| Gene Full Name | catenin delta 1 |
| Gene Summary | This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined. Read-through transcription also exists between this gene and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene. [provided by RefSeq, Dec 2010] |
| Alternative Names | Cadherin associated Src substrate antibody Cadherin-associated Src substrate antibody CAS antibody Catenin (cadherin associated protein) delta 1 antibody Catenin delta 1 antibody Catenin delta antibody Catenin delta-1 antibody CTND1_HUMAN antibody CTNND 1 antibody CTNND antibody Cadherin associated Src substrate antibody Cadherin-associated Src substrate antibody CAS antibody Catenin (cadherin associated protein) delta 1 antibody Catenin delta 1 antibody Catenin delta antibody Catenin delta-1 antibody CTND1_HUMAN antibody CTNND 1 antibody CTNND antibody CTNND1 antibody delta 1 Catenin antibody KIAA0384 antibody p120 antibody P120 CAS antibody p120 catenin antibody P120 CTN antibody p120(cas) antibody p120(ctn) antibody P120CAS antibody P120CTN antibody |
| Molecular Weight(MW) | 108kDa(Observed band size:95kDa) |
| Cellular Localization | Cytoplasm, Cell membrane, Nucleus. |
WB
Western blot analysis of delta 1 Catenin/CAS on SK-Br-3 cell lysates with Rabbit anti-delta 1 Catenin/CAS antibody at 1/1000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-delta 1 Catenin/CAS antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Application: IF-Tissue, Species: Human, Site: liver cancer, Sample: Paraffin-embedded section, Antibody concentration: 1/500.| Application Notes | WB:1:1000 IHC:1:500-1:2000 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
