WB
Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody at 1/2000 dilution. Lane 1: HCT 116-si NT cell lysate, Lane 2: HCT 116-si Glutathione Reductase cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2000 dilution. Positive control: Lane 1: A549, Lane 2: Rat liver tissue.ICC/IF
ICC staining GLUR in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLUR antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUR antibody. Counter stained with hematoxylin.| Product Name | Glutathione Reductase Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human GLUR aa 273-446 / 522. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSR |
|---|---|
| Gene Synonyms | GR GSRD HEL-75 CNSHA10 HEL-S-122m |
| Gene Full Name | glutathione-disulfide reductase |
| Gene Summary | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010] |
| Alternative Names | GLR1 antibody GLUR antibody Glutathione reductase antibody Glutathione reductase mitochondrial antibody Glutathione reductase mitochondrial antibody GR antibody Gr1 antibody GRase antibody GRD 1 antibody GRD1 antibody GLR1 antibody GLUR antibody Glutathione reductase antibody Glutathione reductase mitochondrial antibody Glutathione reductase mitochondrial antibody GR antibody Gr1 antibody GRase antibody GRD 1 antibody GRD1 antibody GSHR_HUMAN antibody GSR antibody MGC78522 antibody |
| Molecular Weight(MW) | 56kDa(Observed band size:54kDa) |
| Cellular Localization | Cytoplasm. Mitochondrion. |
WB
Western blot analysis of Glutathione Reductase on different lysates with Rabbit anti-Glutathione Reductase antibody at 1/2000 dilution. Lane 1: HCT 116-si NT cell lysate, Lane 2: HCT 116-si Glutathione Reductase cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2000 dilution. Positive control: Lane 1: A549, Lane 2: Rat liver tissue.
ICC/IF
ICC staining GLUR in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLUR antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUR antibody. Counter stained with hematoxylin.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
