WB
Western blot analysis of Phospho-PLC-gamma-1 (Y783) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (Y783) antibody at 1/1000 dilution and competitor's antibody at 1/1000 dilution. Lane 1: A431 cell lysate, Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate, Lane 5: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-PLC-gamma-1 (Y783) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLC-gamma-1. |
| Modification | P-Y783 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCG1 |
|---|---|
| Gene Synonyms | IDAA PLC1 NCKAP3 PLC-II PLC148 PLCgamma1 |
| Gene Full Name | phospholipase C gamma 1 |
| Gene Summary | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-1 antibody Inositoltrisphosphohydrolase antibody Monophosphatidylinositol phosphodiesterase antibody NCKAP3 antibody Phosphatidylinositol phospholipase C antibody Phosphoinositidase C antibody Phosphoinositide phospholipase C antibody Phosphoinositide phospholipase C-gamma-1 antibody 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-1 antibody Inositoltrisphosphohydrolase antibody Monophosphatidylinositol phosphodiesterase antibody NCKAP3 antibody Phosphatidylinositol phospholipase C antibody Phosphoinositidase C antibody Phosphoinositide phospholipase C antibody Phosphoinositide phospholipase C-gamma-1 antibody Phospholipase C 148 antibody Phospholipase C gamma 1 antibody Phospholipase C-gamma-1 antibody Phospholipase C-II antibody PLC gamma 1 antibody PLC II antibody PLC-148 antibody PLC-gamma-1 antibody PLC-II antibody PLC1 antibody PLC148 antibody Plcg1 antibody PLCG1_HUMAN antibody PLCgamma1 antibody |
| Molecular Weight(MW) | 149kDa(Observed band size:149kDa) |
| Cellular Localization | Cell projection, lamellipodium, ruffle. |
WB
Western blot analysis of Phospho-PLC-gamma-1 (Y783) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (Y783) antibody at 1/1000 dilution and competitor's antibody at 1/1000 dilution. Lane 1: A431 cell lysate, Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate, Lane 5: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
