WB
Western blot analysis of EHMT2 / G9A on different lysates with Rabbit anti-EHMT2 / G9A antibody at 1/1000 dilution. Lane 1: HAP1-parental cell lysate, Lane 2: HAP1-EHMT2 / G9A KD cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-EHMT2 / G9A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | EHMT2 / G9A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human G9A aa 1,060-1210 / 1210. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:50-1:400 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EHMT2 |
|---|---|
| Gene Synonyms | G9A BAT8 GAT8 NG36 KMT1C C6orf30 |
| Gene Full Name | euchromatic histone lysine methyltransferase 2 |
| Gene Summary | This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
| Alternative Names | Ankyrin repeat containing protein antibody Bat 8 antibody Bat8 antibody C6orf30 antibody DKFZp686H08213 antibody EHMT 2 antibody Ehmt2 antibody EHMT2_HUMAN antibody Euchromatic histone lysine methyltransferase 2 antibody Euchromatic histone lysine N methyltransferase 2 antibody Ankyrin repeat containing protein antibody Bat 8 antibody Bat8 antibody C6orf30 antibody DKFZp686H08213 antibody EHMT 2 antibody Ehmt2 antibody EHMT2_HUMAN antibody Euchromatic histone lysine methyltransferase 2 antibody Euchromatic histone lysine N methyltransferase 2 antibody Euchromatic histone-lysine N-methyltransferase 2 antibody FLJ35547 antibody G 9a antibody G9 a antibody G9a protein antibody G9A antibody G9A histone methyltransferase antibody GAT8 antibody H3 K9 HMTase 3 antibody H3-K9-HMTase 3 antibody Histone H3 K9 methyltransferase 3 antibody Histone H3 K9 methyltransferase3 antibody Histone H3-K9 methyltransferase 3 antibody Histone lysine N methyltransferase antibody Histone lysine N methyltransferase EHMT2 antibody Histone lysine N methyltransferase H3 lysine 9 specific 3 antibody Histone lysine N methyltransferase H3 lysine 9 specific3 antibody Histone-lysine N-methyltransferase EHMT2 antibody HLA B associated transcript 8 antibody HLA-B-associated transcript 8 antibody KMT 1C antibody KMT1 C antibody Lysine N methyltransferase 1C antibody Lysine N-methyltransferase 1C antibody NG 36 antibody NG36 antibody Protein G9a antibody |
| Molecular Weight(MW) | 132kDa(Observed band size:160/170kDa) |
| Cellular Localization | Nucleus, chromosome. |
WB
Western blot analysis of EHMT2 / G9A on different lysates with Rabbit anti-EHMT2 / G9A antibody at 1/1000 dilution. Lane 1: HAP1-parental cell lysate, Lane 2: HAP1-EHMT2 / G9A KD cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-EHMT2 / G9A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
