WB
Western blot analysis of GABA Transporter 1 / GAT 1 on different lysates with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/1000 dilution. Lane 1: Mouse cerebellum tissue lysate, Lane 2: Mouse cerebellum tissue lysate (70℃ heat), Lane 3: Mouse brain tissue lysate, Lane 4: Mouse brain tissue lysate (70℃ heat), Lane 5: Rat brain tissue lysate, Lane 6: Rat brain tissue lysate (70℃ heat), Lysates/proteins at 30 µg/Lane. Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100000 dilution was used for 1 hour at room temperature.IF-F
Application: IHC-Fr, Species: Mouse, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1:1000, Antigen retrieval: Not required.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Application: IF-tissue, Species: Mouse, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1:500.IF-P
Application: IF-tissue, Species: Rat, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1:500.IF-P
Application: IF-tissue, Species: Human, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1:500.| Product Name | GABA Transporter 1 / GAT 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-52. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:1000 IHC:1:500-1:2000 IF-F:1:1000 IF-P:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC6A1 |
|---|---|
| Gene Synonyms | MAE GAT1 GABATR hGAT-1 GABATHG |
| Gene Full Name | solute carrier family 6 member 1 |
| Gene Summary | The protein encoded by this gene is a gamma-aminobutyric acid (GABA) transporter that localizes to the plasma membrane. The encoded protein removes GABA from the synaptic cleft, restoring it to presynaptic terminals. [provided by RefSeq, Jan 2017] |
| Alternative Names | GABATHG antibody GABATR antibody GABT 1 antibody GABT1 antibody GAT-1 antibody GAT1 antibody SC6A1_HUMAN antibody Slc6a1 antibody Sodium and chloride dependent GABA transporter 1 antibody Sodium- and chloride-dependent GABA transporter 1 antibody GABATHG antibody GABATR antibody GABT 1 antibody GABT1 antibody GAT-1 antibody GAT1 antibody SC6A1_HUMAN antibody Slc6a1 antibody Sodium and chloride dependent GABA transporter 1 antibody Sodium- and chloride-dependent GABA transporter 1 antibody Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody Solute carrier family 6 member 1 antibody |
| Molecular Weight(MW) | 67kDa(Observed band size:67kDa) |
| Cellular Localization | Cell membrane, Presynapse. |
WB
Western blot analysis of GABA Transporter 1 / GAT 1 on different lysates with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/1000 dilution. Lane 1: Mouse cerebellum tissue lysate, Lane 2: Mouse cerebellum tissue lysate (70℃ heat), Lane 3: Mouse brain tissue lysate, Lane 4: Mouse brain tissue lysate (70℃ heat), Lane 5: Rat brain tissue lysate, Lane 6: Rat brain tissue lysate (70℃ heat), Lysates/proteins at 30 µg/Lane. Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100000 dilution was used for 1 hour at room temperature.
IF-F
Application: IHC-Fr, Species: Mouse, Site: Cerebellum, Sample: Frozen section, Antibody concentration: 1:1000, Antigen retrieval: Not required.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/2000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Application: IF-tissue, Species: Mouse, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1:500.
IF-P
Application: IF-tissue, Species: Rat, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1:500.
IF-P
Application: IF-tissue, Species: Human, Site: Cerebellum, Sample: Paraffin-embedded section, Antibody concentration: 1:500.| Application Notes | WB:1:1000 IHC:1:500-1:2000 IF-F:1:1000 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
