WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LDLR antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2.IHC
Mouse liver was stained with anti-LDLR Rabbit antibody.| Product Name | LDLR Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:1000-1:4000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LDLR |
|---|---|
| Gene Synonyms | FH FHC FHCL1 LDLCQ2 |
| Gene Full Name | low density lipoprotein receptor |
| Gene Summary | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2022] |
| Molecular Weight(MW) | 95kDa(Observed MW 130kDa) |
| Cellular Localization | Cell membrane ; Single-pass type I membrane protein . Membrane, clathrin-coated pit . Golgi apparatus . Early endosome . Late endosome . Lysosome . Rapidly endocytosed upon ligand binding. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LDLR antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2.
IHC
Mouse liver was stained with anti-LDLR Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
