WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IL-8 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1 Lane 2: THP-1 was treated with TPA(80 nM) for 24 hours and LPS(1 μg/ml) for 15 minutes.ICC/IF
Immunofluorescence analysis of Hela cell. 1,IL-8 Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.ICC/IF
Immunofluorescence analysis of human-liver-cancer tissue. 1,IL-8 Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Product Name | IL-8 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCL8 |
|---|---|
| Gene Synonyms | IL8 NAF GCP1 LECT LUCT NAP1 GCP-1 LYNAP MDNCF MONAP NAP-1 SCYB8 |
| Gene Full Name | C-X-C motif chemokine ligand 8 |
| Gene Summary | The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, May 2020] |
| Alternative Names | IL8 CXCL8 Interleukin-8 IL-8 C-X-C motif chemokine 8 Emoctakin Granulocyte chemotactic protein 1 GCP-1 Monocyte-derived neutrophil chemotactic factor MDNCF Monocyte-derived neutrophil-activating peptide MONAP Neutrophil-activati |
| Molecular Weight(MW) | 11kDa(Observed MW 11kDa) |
| Cellular Localization | Secreted. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IL-8 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1 Lane 2: THP-1 was treated with TPA(80 nM) for 24 hours and LPS(1 μg/ml) for 15 minutes.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,IL-8 Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
ICC/IF
Immunofluorescence analysis of human-liver-cancer tissue. 1,IL-8 Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Application Notes | WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
