WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Podoplanin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: Rat brain.IHC
Mouse lung was stained with anti-Podoplanin rabbit antibody.IHC
Rat lung was stained with anti-Podoplanin rabbit antibody.IHC
Human lung squamous carcinoma was stained with anti-Podoplanin rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Podoplanin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | Podoplanin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 ICC/IF:1:200-1:1000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDPN |
|---|---|
| Gene Synonyms | T1A GP36 GP40 Gp38 OTS8 T1A2 TI1A D2-40 T1A-2 AGGRUS HT1A-1 PA2.26 |
| Gene Full Name | podoplanin |
| Gene Summary | This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | Podoplanin Aggrus Glycoprotein 36 Gp36 PA2.26 antigen T1-alpha T1A |
| Molecular Weight(MW) | 17kDa(Observed MW 36kDa) |
| Cellular Localization | Membrane, Cytoplasm |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Podoplanin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: Rat brain.
IHC
Mouse lung was stained with anti-Podoplanin rabbit antibody.
IHC
Rat lung was stained with anti-Podoplanin rabbit antibody.
IHC
Human lung squamous carcinoma was stained with anti-Podoplanin rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Podoplanin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 ICC/IF:1:200-1:1000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
