WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TBK1 (Phospho Ser172) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela serum was starved overnight and treated with 20% FBS and Calyculin A (100nM) for 15 minutes Lane 3: C6 Lane 4: C6 was treated with Calyculin A(100nM) for 30 minutes.| Product Name | TBK1 (Phospho Ser172) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | BovineHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TBK1 |
|---|---|
| Gene Synonyms | NAK T2K AIARV IIAE8 FTDALS4 |
| Gene Full Name | TANK binding kinase 1 |
| Gene Summary | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response. [provided by RefSeq, Sep 2021] |
| Alternative Names | Serine/threonine-protein kinase TBK1 NF-kappa-B-activating kinase T2K TANK-binding kinase 1 |
| Molecular Weight(MW) | 84kDa(Observed MW 84kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TBK1 (Phospho Ser172) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela serum was starved overnight and treated with 20% FBS and Calyculin A (100nM) for 15 minutes Lane 3: C6 Lane 4: C6 was treated with Calyculin A(100nM) for 30 minutes.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
