WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IRS-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: C2C12 Lane 3: A549.ICC/IF
Immunofluorescence analysis of rat-kidney tissue. 1,IRS-1 Monoclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.ICC/IF
mmunofluorescence analysis of mouse-lung tissue. 1,IRS-1 Monoclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: IRS-1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.IHC
Rat pancreas was stained with anti-IRS-1 rabbit antibody.IHC
Human pancreas was stained with anti-IRS-1 rabbit antibody.| Product Name | IRS-1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:400-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IRS1 |
|---|---|
| Gene Synonyms | HIRS-1 |
| Gene Full Name | insulin receptor substrate 1 |
| Gene Summary | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009] |
| Alternative Names | IRS1 Insulin receptor substrate 1 IRS-1 |
| Molecular Weight(MW) | 131kDa(Observed MW 180kDa) |
| Cellular Localization | nucleus,cytoplasm,cytosol,plasma membrane,insulin receptor complex,caveola,intracellular membrane-bounded organelle. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IRS-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: C2C12 Lane 3: A549.
ICC/IF
Immunofluorescence analysis of rat-kidney tissue. 1,IRS-1 Monoclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
ICC/IF
mmunofluorescence analysis of mouse-lung tissue. 1,IRS-1 Monoclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: IRS-1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.
IHC
Rat pancreas was stained with anti-IRS-1 rabbit antibody.
IHC
Human pancreas was stained with anti-IRS-1 rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:400-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
