WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD86 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: J774A.1.ICC/IF
Immunofluorescence analysis of paraffin-embedded Mouse spleen. Primary Antibody was diluted at 1:200(4° overnight). an Multi colour-Fluorescence kit (Immunoway). EDTA based antigen retrieval was used before Green tyramide signal amplification. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).IHC
Mouse spleen was stained with anti-CD86 rabbit antibody.| Product Name | CD86 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-5000 IHC:1:200-1000 ICC/IF:1:200-1000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD86 |
|---|---|
| Gene Synonyms | B70 B7-2 B7.2 BU63 FUN-1 LAB72 CD28LG2 CD86 v6 |
| Gene Full Name | CD86 molecule |
| Gene Summary | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| Alternative Names | T-lymphocyte activation antigen CD86 Activation B7-2 antigen B70 BU63 CTLA-4 counter-receptor B7.2 FUN-1 CD antigen CD86 |
| Molecular Weight(MW) | 35kDa(Observed MW 60-85kDa) |
| Cellular Localization | Membranous. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD86 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: J774A.1.
ICC/IF
Immunofluorescence analysis of paraffin-embedded Mouse spleen. Primary Antibody was diluted at 1:200(4° overnight). an Multi colour-Fluorescence kit (Immunoway). EDTA based antigen retrieval was used before Green tyramide signal amplification. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
IHC
Mouse spleen was stained with anti-CD86 rabbit antibody.| Application Notes | WB:1:500-5000 IHC:1:200-1000 ICC/IF:1:200-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
