WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β Catenin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT116 Lane 2: Hela Lane 3: C6 Lane 4: U14.WB
Western blot analysis of lysates from HCT116 WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with β Catenin rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human tonsil was stained with anti-β Catenin rabbit antibody.IHC
Mouse stomach was stained with anti-β Catenin rabbit antibody.IHC
Rat jejunum was stained with anti-β Catenin Rabbit antibody.ICC/IF
Rat jejunum was stained with anti-β Catenin Rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: β Catenin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | β Catenin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CTNNB1 |
|---|---|
| Gene Synonyms | EVR7 CTNNB MRD19 NEDSDV armadillo |
| Gene Full Name | catenin beta 1 |
| Gene Summary | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] |
| Alternative Names | CTNNB1 CTNNB OK/SW-cl.35 Catenin beta-1 Beta-catenin |
| Molecular Weight(MW) | 85kDa(Observed MW 85kDa) |
| Cellular Localization | Cytoplasm, Membrane |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β Catenin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT116 Lane 2: Hela Lane 3: C6 Lane 4: U14.
WB
Western blot analysis of lysates from HCT116 WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with β Catenin rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human tonsil was stained with anti-β Catenin rabbit antibody.
IHC
Mouse stomach was stained with anti-β Catenin rabbit antibody.
IHC
Rat jejunum was stained with anti-β Catenin Rabbit antibody.
ICC/IF
Rat jejunum was stained with anti-β Catenin Rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: β Catenin antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
