WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Polycomb Protein SUZ12 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:A549 Lane 2: C2C12 Lane 3: MCF7 Lane 4: L6.IHC
Human kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.IHC
Mouse kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.IHC
Rat kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.ICC/IF
rat kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.| Product Name | Polycomb Protein SUZ12 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SUZ12 |
|---|---|
| Gene Synonyms | CHET9 IMMAS JJAZ1 |
| Gene Full Name | SUZ12 polycomb repressive complex 2 subunit |
| Gene Summary | This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009] |
| Alternative Names | SUZ12 CHET9 JJAZ1 KIAA0160 Polycomb protein SUZ12 Chromatin precipitated E2F target 9 protein ChET 9 protein Joined to JAZF1 protein Suppressor of zeste 12 protein homolog |
| Molecular Weight(MW) | 83kDa(Observed MW 83kDa) |
| Cellular Localization | Nucleus |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Polycomb Protein SUZ12 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:A549 Lane 2: C2C12 Lane 3: MCF7 Lane 4: L6.
IHC
Human kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.
IHC
Mouse kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.
IHC
Rat kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.
ICC/IF
rat kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
