WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD163 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen.WB
Western blot analysis of lysates from SU-DHL-1 WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with CD163 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Mouse liver was stained with anti-CD163 rabbit antibody.IHC
Rat liver was stained with anti-CD163 rabbit antibody.IHC
Human liver was stained with anti-CD163 rabbit antibody.| Product Name | CD163 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD163 |
|---|---|
| Gene Synonyms | M130 MM130 SCARI1 |
| Gene Full Name | CD163 molecule |
| Gene Summary | The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
| Molecular Weight(MW) | 125kDa(Observed MW 250kDa) |
| Cellular Localization | Membranous, Cytoplasmic |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD163 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen.
WB
Western blot analysis of lysates from SU-DHL-1 WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with CD163 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Mouse liver was stained with anti-CD163 rabbit antibody.
IHC
Rat liver was stained with anti-CD163 rabbit antibody.
IHC
Human liver was stained with anti-CD163 rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
