WB
Western blot analysis of TBX18 using anti-TBX18 antibody The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TBX18 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate .IHC
IHC analysis of TBX18 using anti-TBX18 antibody. TBX18 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-TBX18 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of TBX18 using anti-TBX18 antibody. TBX18 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-TBX18 Antibody at a dilution of 1:100. Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody.FC
Flow Cytometry analysis of U87 cells using anti-TBX18 antibody. Overlay histogram showing U87 cells stained with anti-TBX18 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBX18 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-TBX18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human TBX18 recombinant protein (Position: M1-V607). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBX18 |
|---|---|
| Gene Synonyms | PUJO CAKUT2 |
| Gene Full Name | T-box transcription factor 18 |
| Gene Summary | This genes codes for a member of an evolutionarily conserved family of transcription factors that plays a crucial role in embryonic development. The family is characterized by the presence of the DNA-binding T-box domain and is divided into five sub-families based on sequence conservation in this domain. The encoded protein belongs to the vertebrate specific Tbx1 sub-family. The protein acts as a transcriptional repressor by antagonizing transcriptional activators in the T-box family. The protein forms homo- or heterodimers with other transcription factors of the T-box family or other transcription factors. [provided by RefSeq, Nov 2012] |
| Alternative Names | T box 18 T box protein 18 TBX18 |
| Molecular Weight(MW) | 65kDa(Observed MW 70kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of TBX18 using anti-TBX18 antibody The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TBX18 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate .
IHC
IHC analysis of TBX18 using anti-TBX18 antibody. TBX18 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-TBX18 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of TBX18 using anti-TBX18 antibody. TBX18 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-TBX18 Antibody at a dilution of 1:100. Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody.
FC
Flow Cytometry analysis of U87 cells using anti-TBX18 antibody. Overlay histogram showing U87 cells stained with anti-TBX18 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBX18 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
