WB
Flow Cytometry analysis of C6 cells using anti-HNRNPH1 antibody. Overlay histogram showing C6 cells stained with anti-HNRNPH1 antibody (BlueWestern blot analysis of S100A10 using anti-S100A10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: monkey lung tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100A10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.IHC
IHC analysis of S100A10 using anti-S100A10 antibody. S100A10 was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-S100A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of S100A10 using anti-S100A10 antibody. S100A10 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-S100A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).ICC/IF
ICC/IF analysis of S100A10 using anti-S100A10 antibody. S100A10 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-S100A10 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).FC
Flow Cytometry analysis of A431 cells using anti-S100A10 antibody. Overlay histogram showing A431 cells stained with A02787-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A10 Antibody t 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Anti-S100A10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human S100A10 recombinant protein (Position: Q4-K94). Human S100A10 shares 91.2% amino acid (aa) sequence identity with both mouse and rat S100A10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S100A10 |
|---|---|
| Gene Synonyms | 42C P11 p10 GP11 ANX2L CAL1L CLP11 Ca[1] ANX2LG |
| Gene Full Name | S100 calcium binding protein A10 |
| Gene Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq, Jul 2008] |
| Alternative Names | 42C ANX2L ANX2LG CAL1L Calpactin 1 light chain Calpactin I light chain Cellular ligand of annexin II CLP11 GP11 p10 p10 protein P11 Protein S100 A10 S100A10 |
| Molecular Weight(MW) | 11kDa(Observed MW 11kDa) |
| Cellular Localization | cytoplasm |
WB
Flow Cytometry analysis of C6 cells using anti-HNRNPH1 antibody. Overlay histogram showing C6 cells stained with anti-HNRNPH1 antibody (BlueWestern blot analysis of S100A10 using anti-S100A10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: monkey lung tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100A10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IHC
IHC analysis of S100A10 using anti-S100A10 antibody. S100A10 was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-S100A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of S100A10 using anti-S100A10 antibody. S100A10 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-S100A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
ICC/IF
ICC/IF analysis of S100A10 using anti-S100A10 antibody. S100A10 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-S100A10 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
FC
Flow Cytometry analysis of A431 cells using anti-S100A10 antibody. Overlay histogram showing A431 cells stained with A02787-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A10 Antibody t 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
