WB
Western blot analysis of ECHS1 using anti-ECHS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: HT1080 whole cell lysates, Lane 4: 293T whole cell lysates, Lane 5: MCF-7 whole cell lysates, Lane 6: U-87MG whole cell lysates, Lane 7: Caco-2 whole cell lysates, Lane 8: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.WB
Western blot analysis of ECHS1 using anti-ECHS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ECHS1 using anti-ECHS1 antibody. ECHS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ECHS1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IF-P
IF analysis using anti- ECHS1 antibody. detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).IF-P
IF analysis using anti- ECHS1 antibody. detected in paraffin-embedded section of human lung cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).ICC/IF
ICC/IF analysis of ECHS1 using anti-ECHS1 antibody. ECHS1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-ECHS1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of 293T cells using anti-ECHS1 antibody. Overlay histogram showing 293T cells stained with A04508-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECHS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-ECHS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ECHS1 recombinant protein (Position: R12-Q290). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ECHS1 |
|---|---|
| Gene Synonyms | SCEH mECH mECH1 ECHS1D |
| Gene Full Name | enoyl-CoA hydratase, short chain 1 |
| Gene Summary | The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008] |
| Alternative Names | ECHS1 ECoH-1 Enoyl CoA hydratase 1 SCEH |
| Molecular Weight(MW) | 29kDa(Observed MW 29kDa) |
| Cellular Localization | Mitochondrion matrix |
WB
Western blot analysis of ECHS1 using anti-ECHS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: HT1080 whole cell lysates, Lane 4: 293T whole cell lysates, Lane 5: MCF-7 whole cell lysates, Lane 6: U-87MG whole cell lysates, Lane 7: Caco-2 whole cell lysates, Lane 8: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
WB
Western blot analysis of ECHS1 using anti-ECHS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ECHS1 using anti-ECHS1 antibody. ECHS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ECHS1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IF-P
IF analysis using anti- ECHS1 antibody. detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
IF-P
IF analysis using anti- ECHS1 antibody. detected in paraffin-embedded section of human lung cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
ICC/IF
ICC/IF analysis of ECHS1 using anti-ECHS1 antibody. ECHS1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-ECHS1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of 293T cells using anti-ECHS1 antibody. Overlay histogram showing 293T cells stained with A04508-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECHS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
