WB
Western blot analysis of MED4 using anti-MED4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates, Lane 8: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .IHC
IHC analysis of MED4 using anti-MED4 antibody . MED4 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-MED4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of MED4 using anti-MED4 antibody and anti-Beta Tubulin antibody. MED4 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-MED4 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Fluoro550-conjugated Anti- mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of Jurkat cells using anti-MED4 antibody. Overlay histogram showing Jurkat cells stained with anti-MED4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Anti-MED4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human MED4 recombinant protein (Position: R27-H247). Human MED4 shares 96.8% and 96.4% amino acid (aa) sequence identity with mouse and rat MED4, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED4 |
|---|---|
| Gene Synonyms | ARC36 VDRIP DRIP36 TRAP36 HSPC126 |
| Gene Full Name | mediator complex subunit 4 |
| Gene Summary | This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| Alternative Names | ARC36 DRIP36 FLJ10956 HSPC126 MED4 mediator complex subunit 4 RP11 90M2.2 TRAP36 VDRIP |
| Molecular Weight(MW) | 30kDa(Observed MW 35kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of MED4 using anti-MED4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates, Lane 8: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .
IHC
IHC analysis of MED4 using anti-MED4 antibody . MED4 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-MED4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of MED4 using anti-MED4 antibody and anti-Beta Tubulin antibody. MED4 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-MED4 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Fluoro550-conjugated Anti- mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of Jurkat cells using anti-MED4 antibody. Overlay histogram showing Jurkat cells stained with anti-MED4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
