WB
Western blot analysis of mDia1/DIAPH1 using anti-mDia1/DIAPH1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: Raji whole cell lysates, Lane 3: Hela whole cell lysates, Lane 4: COS-7 whole cell lysates, Lane 5: Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-mDia1/DIAPH1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of mDia1/DIAPH1 using anti-mDia1/DIAPH1 antibody. mDia1/DIAPH1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-mDia1/DIAPH1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of K562 cells using anti-mDia1/DIAPH1 antibody. Overlay histogram showing K562 cells stained with anti-mDia1/DIAPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-mDia1/DIAPH1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-mDia1/DIAPH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human DIAPH1 recombinant protein (Position: D1039-Q1153). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIAPH1 |
|---|---|
| Gene Synonyms | DIA1 DRF1 DFNA1 LFHL1 SCBMS hDIA1 mDia1 |
| Gene Full Name | diaphanous related formin 1 |
| Gene Summary | This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | DFNA1 DIA1 DIAP1 DIAPH1 Diaphanous related formin 1 hDIA1 mDia1 Protein diaphanous homolog 1 |
| Molecular Weight(MW) | 141kDa(Observed MW 150kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of mDia1/DIAPH1 using anti-mDia1/DIAPH1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: Raji whole cell lysates, Lane 3: Hela whole cell lysates, Lane 4: COS-7 whole cell lysates, Lane 5: Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-mDia1/DIAPH1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of mDia1/DIAPH1 using anti-mDia1/DIAPH1 antibody. mDia1/DIAPH1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-mDia1/DIAPH1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of K562 cells using anti-mDia1/DIAPH1 antibody. Overlay histogram showing K562 cells stained with anti-mDia1/DIAPH1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-mDia1/DIAPH1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
