WB
Western blot analysis of TFAP2C using anti-TFAP2C antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFAP2C antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of TFAP2C using anti-TFAP2C antibody. TFAP2C was detected in a paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFAP2C Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of TFAP2C using anti-TFAP2C antibody. TFAP2C was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-TFAP2C Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Anti-TFAP2C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human AP2 gamma/TFAP2C recombinant protein (Position: M1-N219). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-2000 ICC/IF:1:50-400 IHC:1:50-400 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFAP2C |
|---|---|
| Gene Synonyms | ERF1 TFAP2G hAP-2g AP2-GAMMA |
| Gene Full Name | transcription factor AP-2 gamma |
| Gene Summary | The protein encoded by this gene is a sequence-specific DNA-binding transcription factor involved in the activation of several developmental genes. The encoded protein can act as either a homodimer or heterodimer with other family members and is induced during retinoic acid-mediated differentiation. It plays a role in the development of the eyes, face, body wall, limbs, and neural tube. [provided by RefSeq, Jul 2008] |
| Alternative Names | ap 2gamma AP2 GAMMA ERF1 hAP 2g TFAP2C TFAP2G Transcription factor ERF 1 |
| Molecular Weight(MW) | 49kDa(Observed MW 49kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of TFAP2C using anti-TFAP2C antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFAP2C antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of TFAP2C using anti-TFAP2C antibody. TFAP2C was detected in a paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TFAP2C Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of TFAP2C using anti-TFAP2C antibody. TFAP2C was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-TFAP2C Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 IHC:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
