WB
Western blot analysis of various lysates using DiMethyl-Histone H4-K20 Rabbit pAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffinembedded Rat testis using DiMethyl-HistoneH4-K20 Rabbit pAb at dilution of1:200 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis using DiMethylHistone H4-K20 Rabbit pAb at dilution of 1:200 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.ICC/IF
Immunofluorescence analysis of 293T cellsusing DiMethyl-Histone H4-K20 Rabbit pAb. Blue: DAPI for nuclear staining.| Product Name | DiMethyl-Histone H4-K20 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4FA HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 11kDa(Observed band size:11kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using DiMethyl-Histone H4-K20 Rabbit pAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat testis using DiMethyl-HistoneH4-K20 Rabbit pAb at dilution of1:200 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse testis using DiMethylHistone H4-K20 Rabbit pAb at dilution of 1:200 (40x lens). Microwaveantigen retrieval performed with 0.01M PBSBuffer (pH 7.2) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of 293T cellsusing DiMethyl-Histone H4-K20 Rabbit pAb. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
