WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IGF1 Receptor (Phospho Tyr1135/1136)/Insulin Receptor β (Phospho Tyr1150/1151) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 serum was starved for 24 hours and then treated with IGF-1 (100ng/ml) for 20 minutes Lane 3: C6 Lane 4: C6 serum was starved for 24 hours and then treated with IGF-1 (100ng/ml) for 20 minutes.| Product Name | IGF-IR (pTyr1135/6)/INSR β (pTyr1150/1) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Phospho |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IGF1R |
|---|---|
| Gene Synonyms | IGFR CD221 IGFIR JTK13 |
| Gene Full Name | insulin like growth factor 1 receptor |
| Gene Summary | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| Alternative Names | IGF1R Insulin-like growth factor 1 receptor Insulin-like growth factor I receptor IGF-I receptor CD antigen CD221 INSR Insulin receptor IR CD antigen CD220 |
| Molecular Weight(MW) | 156kDa(Observed MW 95kDa) |
| Cellular Localization | Cell membrane ; Single-pass type I membrane protein . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IGF1 Receptor (Phospho Tyr1135/1136)/Insulin Receptor β (Phospho Tyr1150/1151) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 serum was starved for 24 hours and then treated with IGF-1 (100ng/ml) for 20 minutes Lane 3: C6 Lane 4: C6 serum was starved for 24 hours and then treated with IGF-1 (100ng/ml) for 20 minutes.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
