WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane2: HEK293 - Human normal embryonic kidney Lane3: HepG2 - Human hepatocellular carcinoma Lane4: HCT-116 - Human colon cancer Lane5: A549 - Human lung adenocarcinoma Lane6: A431 - Human skin squamous cell carcinoma Lane7: K562 - Human chronic myeloid leukemia Lane8: Jurkat - Human acute T cell leukemia cells Lane9: MCF7 - Human breast cancer Lane10: SH-SY5Y - Human neuroblastoma cells Lane11: NIH-3T3 - NIH mouse fibroblasts Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells Lane13: C6 - Rat glioma cells Lane14: PC-12 - Pheochromocytoma in rats.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Amyloid-β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: Mouse brain Lane 3: Hela.IHC
Human brain was stained with anti-Amyloid-β rabbit antibody.IHC
Mouse brain was stained with anti-Amyloid-β rabbit antibody.ICC/IF
at brain was stained with anti-Amyloid-β rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,Amyloid-β Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min).ICC/IF
Immunofluorescence analysis of Hela cell. 1,Amyloid-β Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Amyloid Precursor Protein antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | Amyloid-β Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HamsterHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | APP |
|---|---|
| Gene Synonyms | AAA AD1 PN2 ABPP APPI CVAP ABETA PN-II preA4 CTFgamma alpha-sAPP |
| Gene Full Name | amyloid beta precursor protein |
| Gene Summary | This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014] |
| Alternative Names | APP A4 AD1 Amyloid beta A4 protein ABPP APPI APP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide CVAP PreA4 Protease nexin-II PN-II |
| Molecular Weight(MW) | 87kDa(Observed MW 100kDa) |
| Cellular Localization | Membrane |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane2: HEK293 - Human normal embryonic kidney Lane3: HepG2 - Human hepatocellular carcinoma Lane4: HCT-116 - Human colon cancer Lane5: A549 - Human lung adenocarcinoma Lane6: A431 - Human skin squamous cell carcinoma Lane7: K562 - Human chronic myeloid leukemia Lane8: Jurkat - Human acute T cell leukemia cells Lane9: MCF7 - Human breast cancer Lane10: SH-SY5Y - Human neuroblastoma cells Lane11: NIH-3T3 - NIH mouse fibroblasts Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells Lane13: C6 - Rat glioma cells Lane14: PC-12 - Pheochromocytoma in rats.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Amyloid-β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: Mouse brain Lane 3: Hela.
IHC
Human brain was stained with anti-Amyloid-β rabbit antibody.
IHC
Mouse brain was stained with anti-Amyloid-β rabbit antibody.
ICC/IF
at brain was stained with anti-Amyloid-β rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,Amyloid-β Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min).
ICC/IF
Immunofluorescence analysis of Hela cell. 1,Amyloid-β Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Amyloid Precursor Protein antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
