WB
Western Blot analysis using various cell lysate, Proteins were separated by 4-20% SDS-PAGE, and the membrane was blotted with Argonaute-2 Rabbit mAb diluted at 1:2000. Secondary :Dylight 800, Goat Anti Rabbit IgG.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AGO2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: RAW264.7.IHC
Human kidney was stained with anti-AGO2 rabbit antibody.IHC
Rat kidney was stained with anti-AGO2 rabbit antibody.ICC/IF
Immunofluorescence analysis of Human kidney.ICC/IF
Immunofluorescence analysis of Rat kidney.ICC/IF
Immunofluorescence analysis of HeLa . Picture A: AGO2 Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | AGO2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AGO2 |
|---|---|
| Gene Synonyms | PPD Q10 CASC7 EIF2C2 LESKRES LINC00980 |
| Gene Full Name | argonaute RISC catalytic component 2 |
| Gene Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Molecular Weight(MW) | 97kDa(Observed MW 97kDa) |
| Cellular Localization | Cytoplasm, P-body . Nucleus . |
WB
Western Blot analysis using various cell lysate, Proteins were separated by 4-20% SDS-PAGE, and the membrane was blotted with Argonaute-2 Rabbit mAb diluted at 1:2000. Secondary :Dylight 800, Goat Anti Rabbit IgG.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AGO2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: RAW264.7.
IHC
Human kidney was stained with anti-AGO2 rabbit antibody.
IHC
Rat kidney was stained with anti-AGO2 rabbit antibody.
ICC/IF
Immunofluorescence analysis of Human kidney.
ICC/IF
Immunofluorescence analysis of Rat kidney.
ICC/IF
Immunofluorescence analysis of HeLa . Picture A: AGO2 Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:2000-1:10000 IHC:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
