WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TATA Box Binding Protein antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: Hela Lane 3: U-14 Lane 4: Rat womb.WB
Western Blot analysis of various cells using TFIID Antibody.IHC
Human colon was stained with Anti-TATA Box Binding Protein rabbit antibody.IHC
Mouse colon was stained with Anti-TATA Box Binding Protein rabbit antibody.IHC
Rat colon was stained with Anti-TATA Box Binding Protein rabbit antibody.ICC/IF
rat colon was stained with Anti-TATA Box Binding Protein rabbit antibody.ICC/IF
Immunofluorescence analysis of HeLa. Picture A: TATA Box Binding Protein Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | TBP/TATA Box Binding Protein Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-5000 IHC:1:400-1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TBP |
|---|---|
| Gene Synonyms | HDL4 TBP1 GTF2D SCA17 TFIID GTF2D1 |
| Gene Full Name | TATA-box binding protein |
| Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016] |
| Alternative Names | TBP GTF2D1 TF2D TFIID TATA-box-binding protein TATA sequence-binding protein TATA-binding factor TATA-box factor Transcription initiation factor TFIID TBP subunit |
| Molecular Weight(MW) | 38kDa(Observed MW 38kDa) |
| Cellular Localization | Nucleus |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TATA Box Binding Protein antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: Hela Lane 3: U-14 Lane 4: Rat womb.
WB
Western Blot analysis of various cells using TFIID Antibody.
IHC
Human colon was stained with Anti-TATA Box Binding Protein rabbit antibody.
IHC
Mouse colon was stained with Anti-TATA Box Binding Protein rabbit antibody.
IHC
Rat colon was stained with Anti-TATA Box Binding Protein rabbit antibody.
ICC/IF
rat colon was stained with Anti-TATA Box Binding Protein rabbit antibody.
ICC/IF
Immunofluorescence analysis of HeLa. Picture A: TATA Box Binding Protein Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-5000 IHC:1:400-1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
