WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SIRT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Jurkat.WB
Western blot analysis of the lysates from COLO205 cells using SirT1 antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.WB
Western blot analysis of lysates from Jurkat WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with SIRT1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human testis was stained with Anti-SIRT1 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A:SIRT1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.ICC/IF
Immunofluorescence analysis of rat-spleen tissue. 1,SIRT1 Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Product Name | SIRT1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-5000 IHC:1:100-200 ICC/IF:1:200-1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SIRT1 |
|---|---|
| Gene Synonyms | SIR2 SIR2L1 SIR2alpha |
| Gene Full Name | sirtuin 1 |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] |
| Alternative Names | SIRT1 SIR2L1 NAD-dependent protein deacetylase sirtuin-1 hSIRT1 Regulatory protein SIR2 homolog 1 SIR2-like protein 1 hSIR2 |
| Molecular Weight(MW) | 81kDa(Observed MW 130kDa) |
| Cellular Localization | Nuclear |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SIRT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Jurkat.
WB
Western blot analysis of the lysates from COLO205 cells using SirT1 antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture.
WB
Western blot analysis of lysates from Jurkat WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with SIRT1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human testis was stained with Anti-SIRT1 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A:SIRT1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.
ICC/IF
Immunofluorescence analysis of rat-spleen tissue. 1,SIRT1 Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Application Notes | WB:1:1000-5000 IHC:1:100-200 ICC/IF:1:200-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
