WB
Western blot analysis of MAFA using anti-MAFA antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAFA antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of MAFA using anti-MAFA antibody. MAFA was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MAFA Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of MAFA using anti-MAFA antibody. MAFA was detected in a paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MAFA Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of MAFA using anti-MAFA antibody.MAFA was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MAFA Antibody overnight at 4°C. Fluoro594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.FC
Flow Cytometry analysis of SiHa cells using anti-MAFA antibody.Overlay histogram showing SiHa cells stained with anti-MAFA antibody (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAFA Antibody for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Anti-MAFA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human MAFA recombinant protein (Position: A9-D308). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAFA |
|---|---|
| Gene Synonyms | INSDM hMafA RIPE3b1 |
| Gene Full Name | MAF bZIP transcription factor A |
| Gene Summary | MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008] |
| Alternative Names | MAF bZIP transcription factor A INSDM RIPE3b1 hMafA MAFA |
| Molecular Weight(MW) | 37kDa(Observed MW 50kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of MAFA using anti-MAFA antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAFA antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of MAFA using anti-MAFA antibody. MAFA was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MAFA Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of MAFA using anti-MAFA antibody. MAFA was detected in a paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MAFA Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of MAFA using anti-MAFA antibody.MAFA was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MAFA Antibody overnight at 4°C. Fluoro594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FC
Flow Cytometry analysis of SiHa cells using anti-MAFA antibody.Overlay histogram showing SiHa cells stained with anti-MAFA antibody (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAFA Antibody for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
