WB
Western blot analysis of ND6/MT-ND6 using anti-ND6/MT-ND6 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates, Lane 3: RT4 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ND6/MT-ND6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of ND6/MT-ND6 using anti-ND6/MT-ND6 antibody. ND6/MT-ND6 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ND6/MT-ND6 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-ND6/MT-ND6 antibody. Overlay histogram showing HepG2 cells stained with anti-ND6/MT-ND6 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ND6/MT-ND6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-ND6/MT-ND6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human ND6/MT-ND6 recombinant protein (Position: V10-N174). Human MT-ND6 shares 54.7% and 53.2% amino acid (aa) sequence identity with mouse and rat MT-ND6, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MT-ND6 |
|---|---|
| Gene Synonyms | MTND6 ND6 |
| Gene Full Name | mitochondrially encoded NADH dehydrogenase 6 |
| Gene Summary | Enables NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial electron transport, NADH to ubiquinone and mitochondrial respiratory chain complex I assembly. Located in mitochondrial inner membrane. Implicated in Leber hereditary optic neuropathy; Leigh disease; and spinal muscular atrophy with lower extremity predominant 2B. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | MT-ND6 MTND6 NADH6 ND6 NADH-ubiquinone oxidoreductase chain 6 EC 7.1.1.2 NADH dehydrogenase subunit 6 |
| Molecular Weight(MW) | 19kDa(Observed MW 19kDa) |
| Cellular Localization | Mitochondrion inner membrane |
WB
Western blot analysis of ND6/MT-ND6 using anti-ND6/MT-ND6 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates, Lane 3: RT4 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ND6/MT-ND6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of ND6/MT-ND6 using anti-ND6/MT-ND6 antibody. ND6/MT-ND6 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ND6/MT-ND6 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-ND6/MT-ND6 antibody. Overlay histogram showing HepG2 cells stained with anti-ND6/MT-ND6 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ND6/MT-ND6 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
