WB
Western blot analysis of NOGO/RTN4 using anti-NOGO/RTN4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOGO/RTN4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of NOGO/RTN4 using anti-NOGO/RTN4 antibody. NOGO/RTN4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NOGO/RTN4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of NOGO/RTN4 using anti-NOGO/RTN4 antibody. NOGO/RTN4 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NOGO/RTN4 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis using anti-RTN4 antibody. detected in paraffin-embedded section of human prostatic cancer® tissue. The tissue section were stained using the Fluoro488 conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).FC
Flow Cytometry analysis of SiHa cells using anti-NOGO/RTN4 antibody. Overlay histogram showing SiHa cells stained with anti-NOGO/RTN4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOGO/RTN4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-NOGO/RTN4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Nogo A/RTN4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RTN4 |
|---|---|
| Gene Synonyms | ASY NSP NOGO NOGOA NOGOB RTN-X NSP-CL RTN4-A RTN4-C RTN4-B1 RTN4-B2 NI220/250 Nbla00271 Nbla10545 |
| Gene Full Name | reticulon 4 |
| Gene Summary | This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | ASY Foocen KIAA0886 Nbla00271 Nbla10545 Neurite outgrowth inhibitor NI220/250 NOGO NOGO A Nogo B Nogo C Nogo protein NOGOA NOGOC NSP NSP CL reticulon 4 Reticulon 5 RTN X RTN4 RTN4 A RTN4 B1 RTN4 B2 RTN4 C SP1507 |
| Molecular Weight(MW) | 130kDa(Observed MW 42/180kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of NOGO/RTN4 using anti-NOGO/RTN4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOGO/RTN4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of NOGO/RTN4 using anti-NOGO/RTN4 antibody. NOGO/RTN4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NOGO/RTN4 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of NOGO/RTN4 using anti-NOGO/RTN4 antibody. NOGO/RTN4 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NOGO/RTN4 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis using anti-RTN4 antibody. detected in paraffin-embedded section of human prostatic cancer® tissue. The tissue section were stained using the Fluoro488 conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of SiHa cells using anti-NOGO/RTN4 antibody. Overlay histogram showing SiHa cells stained with anti-NOGO/RTN4 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOGO/RTN4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 IF-P:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
