WB
Western blot analysis of ADAMTS5 using anti-ADAMTS5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human hela whole cell lysates, Lane 2: Human hepG2 whole cell lysates, Lane 3: Human U2OS whole cell lysates, Lane 4: Human SW620 whole cell lysates, Lane 5: Human THP-1 whole cell lysates, Lane 6: Human Raji whole cell lysates, Lane 7: Human SGC-7901 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADAMTS5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ADAMTS5 using anti-ADAMTS5 antibody. ADAMTS5 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ADAMTS5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Product Name | Anti-ADAMTS5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human ADAMTS5 recombinant protein (Position: D747-K780). Human ADAMTS5 shares 100% amino acid (aa) sequence identity with mouse ADAMTS5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-2000 IHC:1:50-400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAMTS5 |
|---|---|
| Gene Synonyms | ADMP-2 ADAM-TS5 ADAMTS-5 ADAMTS11 ADAM-TS 5 ADAMTS-11 ADAM-TS 11 |
| Gene Full Name | ADAM metallopeptidase with thrombospondin type 1 motif 5 |
| Gene Summary | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and functions as an aggrecanase that cleaves aggrecan, a major proteoglycan of cartilage, and may mediate cartilage destruction in osteoarthritis. [provided by RefSeq, Feb 2016] |
| Alternative Names | ADAM metallopeptidase with thrombospondin type 1 motif 5 ADAM-TS 11 ADAM-TS 5 ADAM-TS5 ADAMTS-11 ADAMTS-5 ADAMTS11 ADMP-2 ADAMTS5 |
| Molecular Weight(MW) | 102kDa(Observed MW 75kDa) |
| Cellular Localization | Secreted |
WB
Western blot analysis of ADAMTS5 using anti-ADAMTS5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human hela whole cell lysates, Lane 2: Human hepG2 whole cell lysates, Lane 3: Human U2OS whole cell lysates, Lane 4: Human SW620 whole cell lysates, Lane 5: Human THP-1 whole cell lysates, Lane 6: Human Raji whole cell lysates, Lane 7: Human SGC-7901 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADAMTS5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ADAMTS5 using anti-ADAMTS5 antibody. ADAMTS5 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ADAMTS5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Application Notes | WB:1:500-2000 IHC:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
